![]() ![]() The first three components of the 3D-FCA ( Fig. The global F ST value indicates that 4.3% of the total allele frequency variance lies among sample sites and is highly significant ( P < 0.0001), while 95.7% is explained by the variation within sites. Fourteen of the 15 tests ( P < 0.05) showed significant pairwise F ST values after correction for multiple comparisons (Holm, 1979 Rice, 1989). Pairwise F ST values among all six locations indicate ( Table 2) that the highest F ST (0.1112) value was between Canal Coldita-Piedra Blanca (2-CB) and Caleta La Arena (1-LA). Information content and the fixation indices F IS, F ST, F IT,Īlthough average relatedness ( r xy) between pairs of individuals from the same site was significantly higher than between pairs of randomly-mixed individuals from different locations, at -0.019 ± 0.0013 and -0.137 ± 0.0009, respectively ( P < 0.0001), neither value was significantly different from zero. Heterozygosity, He: the expected heterozygosity, PIC: polymorphic (n = 300) of blue mussel ( Mytilus chilensis) in southern Chile using ![]() Global genetic diversity estimators by locus in samples Tests for linkage disequilibrium ( LD) showed no significant deviations for any of the 216 tests performed (36 locus combinations x 6 locations) after sequential Bonferroni correction for multiple tests (Holm, 1979 Rice, 1989). Significant deviations corresponded to positive Fis values observed in various loci, indicating heterozygote deficiencies. Significant deviations from HWE were observed in 45 of the 54 tests (9 loci x 6 locations), performed after correction for multiple testing (Holm, 1979 Rice, 1989). Of the analyzed loci, 45 of 54 tests performed deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction ( P 0.5) (He et al., 2012). We found 75 different alleles, six of which were private alleles. Nine polymorphic microsatellite (SSR) loci were genotyped ( Mgu1, Mgu3, MT203, MT282, Mg15, Mg56, Med737, MIT02 and MGE005). The genetic diversity and differentiation of the blue mussel in southern Chile was investigated by sampling six locations: one natural bank and five seed collection centers. Mussels ( Mytilus spp.) are one of the most cultivated and commercialized bivalves in southern Chile culture is currently supplied almost entirely from wild-caught seed obtained from relatively few collection centers. Díaz 2, Carmen Lamas 2, Carla Uribe 2, Felipe Jilberto 2 & Cristián Araneda 2ġDepartamento de Ciencia de los Alimentos y Tecnología Química Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile Sergio Livingstone 1007 Independencia, Santiago, ChileĢDepartamento de Producción Animal, Facultad de Ciencias Agronómicas, Universidad de ChileĬorresponding author: María Angélica Larraín editor: Cristian AldeaĪBSTRACT. Heterologous microsatellite-based genetic diversity in blue mussel ( Mytilus chilensis) and differentiation among localities in southern Chileĭiversidad genética del mejillón ( Mytilus chilensis) y diferenciación entre localidades del sur de Chile usando marcadores microsatélites heterólogos ![]()
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